Factors affecting Hemagglutination Activity of Avian Influenza Virus Subtype H 5 1
نویسندگان
چکیده
Hemagglutination (HA) and hemagglutination inhibition (HI) tests for avian influenza (AI) virus (H5N1) were standardized varying various factors like erythrocytes from different species, type of diluent, incubation temperature and incubation period. The virus was propagated in embryonated chicken eggs (9-11 days). The allantoic fluid (AF) was harvested 36 hours post incubation and was confirmed by spot agglutination test and agar gel precipitation test. The maximum HA titres were obtained using 1% RBCs of chicken, human blood (O) and dog at 22-37 C for 30-40 minutes. The AI virus subtype H5N1 eluted rapidly with higher temperature and maximum elution was observed within 8 hours. The maximum HI titres were obtained using 4 HA units of AI virus antigen compared to 1 or 8 HA units.
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